Related antibodies

B30C7

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Antibody UID: 
ITB01049
Type: 
Monoclonal Antibody
Other Names: 
MCA5973GA
Isotype: 
IgG1
Clone: 
B30C7
Recombinant antibody available: 
No
Commercially available
Target molecule(s):
Pig-CD27
Supplier:
BIO-RAD
Request
BIO-RAD
Porcine anti-porcine CD27

Please submit additional references, images or any other useful information through the comments section.

Flow Cytometry [FC]
5
5 out of 5 1 votes
Immunohistochemistry- Frozen [IHCF]
0
Immunohistochemistry- Paraffin [IHCP]
0
Immunocytochemistry [IHC]
0
Immunoprecipitation [IP]
0
ELISA [E]
0
Western Blotting [WB]
0
Cell function [CF]
0
Affinity sorting [AS]
0
In vivo depletion [IVD]
0
ELISPOT [ES]
0
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Comments

Dr Wilhelm Gerner | Thu, 08/08/2019 - 10:12 #1
Monoclonal antibody b30c7 recognizes porcine CD27 (doi: 10.1016/j.dci.2012.06.011) and can be used in combination with mAbs against CD4 and CD8alpha to identify naïve and central memory CD4 T cells in the pig (doi: 10.1186/1297-9716-44-18). It can also aid in the identification of effector CD8 T cells (absence or reduced expression of CD27) in combination with analysis of perforin or T-bet expression (doi: 10.1016/j.dci.2016.02.022). The staining example shows CD27 expression in combination with CD8alpha on blood-derived porcine lymphocytes. The high fluorescence intensity for CD27 was achieved by labelling with an isotype-specific secondary antibody conjugated with PE (goat-anti mouse IgG1-PE). CD8alpha was labelled with mAb 11/295/33. This antibody was directly conjugated to AlexaFluor647.

References

Reutner K, Leitner J, Essler SE, Witter K, Patzl M, Steinberger P, Saalmüller A, Gerner W.: Porcine CD27: identification, expression and functional aspects in lymphocyte subsets in swine. Dev Comp Immunol, 2012; 38:321-331

link
Katharina Reutner, Judith Leitner, Sabine E. Essler, Kirsti Witter, Martina Patzl, Peter Steinberger, Armin Saalmüller, Wilhelm Gerner: Porcine CD27: Identification, expression and functional aspects in lymphocyte subsets in swine Developmental & Comparative Immunology, 44551; 38:321-331 link
Katharina Reutner, Judith Leitner, Andrea Müllebner, Andrea Ladinig, Sabine E Essler, J Catharina Duvigneau, Mathias Ritzmann, Peter Steinberger, Armin Saalmüller, Wilhelm Gerner: CD27 expression discriminates porcine T helper cells with functionally distinct properties Veterinary Research, 45162; 44:18 link
Irene M. Rodríguez-Gómez, Stephanie C. Talker, Tobias Käser, Maria Stadler, Sabine E. Hammer, Armin Saalmüller, Wilhelm Gerner: Expression of T-bet, Eomesodermin and GATA-3 in porcine αβ T cells Developmental & Comparative Immunology, 48120; 60:115-126 link